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In situ hybridization for P-selectin, leukocyte-specific antigen-I, Mac-1, and CD18 on capillary-like structures in the developing rat CNS. The endothelial cells of the cerebral microvasculature of the rat brain were examined for the expression of P-selectin, a cell surface protein that is a ligand for selectins, and for the expression of leukocyte-specific antigen-I (LSA-I) and beta 2 integrin family members (Mac-1 and CD18). P-selectin was detectable from embryonic day (ED) 10 and was abundant on microvessels from ED 12 to 17. However, a few P-selectin positive microvessels were still seen in the brain on ED 18. On ED 13, LSA-I, Mac-1, and CD18 were expressed on blood vessels in the brain, including microvessels. These molecules were present in microvessels from ED 15 onward. In situ hybridization demonstrated the presence of messenger RNA (mRNA) for these molecules in the endothelial cells of microvessels from ED 13 to 17. The expression of P-selectin on blood vessels in the brain was time dependent and preceded that of Mac-1 and CD18. These findings indicate that some leukocytes may enter the CNS during the early phase of cerebral development.CALL me desperate if you must, but I'd like to think that I'm nothing like Chuck Roven. In a recent interview with MTV, Roven, of all people, gave a brief run-down of the current state of the action film, and how he thinks that we should feel about the genre. According to the director, today's movies are "not meaningful, I think they're irresponsible" – and in Roven's opinion, no action hero should ever again be played by Denzel Washington. So, given that it's "a pretty cynical time", Roven says he's optimistic about the potential of an action hero "with some sort of personal journey, that's a man's man, a man's journey, with great, beautiful lines". You know, like I said, not Denzel. "A great performance would never be about size. It would be about character, and I think what we've seen lately, action films are lacking in character. And I think that's a mistake." I suppose Roven has a point, but it's certainly hard to argue that The


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